Journal: Journal of Neuroinflammation

Article Title: Protection of ischemic post conditioning against transient focal ischemia-induced brain damage is associated with inhibition of neuroinflammation via modulation of TLR2 and TLR4 pathways

doi: 10.1186/1742-2094-11-15

Figure Lengend Snippet: The effect of ischemic postconditioning on TLR2 between 2 hour and 4.5 hour ischemia groups. (A) Representative images of cells with positive immunochemical staining to TLR2, scale bar = 50 μm. (B) Statistics of the number of cells stained with TLR2. (C) Statistics of the transcriptional levels of TLR2. (D) Representative Western blotting images of TLR2. (E) Statistics of the expressional level of TLR2. (F) Representative images under confocal microscope in the sham group. (G) Representative images under confocal microscope in the ischemia group; (n = 4 in each group).

Article Snippet: After the standard histological procedures, sections were incubated overnight at 4°C in the mixture of 1:100 rabbit anti-TLR2 (Boster Biotechnology Company, Wuhan, China)/1:100 mouse anti-NeuN (Millipore Corporation, Billerica, USA), 1:100 rabbit anti-TLR2/1:1,000 mouse anti-GFAP (Millipore Corporation, Billerica, USA), 1:100 rabbit anti-TLR2 (Boster Biotechnology Company, Wuhan, China), 1:100 rabbit anti-TLR4 (Boster Biotechnology Company, Wuhan, China)/1:100 mouse anti-NeuN (Millipore Corporation, Billerica, USA), 1:100 mouse anti-TLR4 (Abcam, Cambridge, USA)/1:1,000 rabbit anti-GFAP (Millipore Corporation, Billerica, USA), 1:100 mouse anti-TLR4 (Abcam, Cambridge, USA).

Techniques: Staining, Western Blot, Microscopy